Imaging

The Telethon Kids institute is equipped with a diverse range of histology and microscopy equipment and analysis software to facilitate state-of-the-art imaging and study of intracellular organelles, cells and tissue.

Bright Blue Cancer Analysis Suite
Generously supported by donations from Bright Blue, the Institute provides a suite of equipment encompassing all aspects of tissue processing for histology and pathology and enabling a wide range of microscopy applications including transmitted light (brightfield, phase contrast, differential interference contrast (DIC) and fluorescence-based techniques such as epifluorescence (with spectral unmixing capabilities) and confocal.

We also have capabilities to allow live cell time-lapse microscopy for short-(hours) or long- (days) term experiments. Together, this equipment enables our researchers to visualise cells or tissue to study cell function and how they are affected by changing environmental, physiological and disease conditions such as cancer.

The facility incorporates a range of manual and automated histology and pathology instruments including:

• Leica Automated Vacuum Tissue processor ASP200S
• Leica Tissue paraffin embedder EG1150
• Leica CM1800 cryostat
• Leica RM2135 microtome
• KD3358 Semi-automatic microtome + floatation workstation
• Leica Autostainer XL

Our microscopes include:

• Nikon C2+ Confocal Microscope 
• 3D Histech Pannoramic Midi Digital Slide Scanner
• Nuance Multispectral Imaging System fitted to a Leica DMLB Fluorescent Microscope
• Olympus BX53 Epifluorescent Microscope Fitted with a Olympus DP72 Camera