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        Flow Facility

        Flow cytometry is a technology used to measure complex cell phenotype and functions including:

        • Cell surface markers
        • Intracellular markers
        • Cytokine secretion
        • Phosphoprotein signalling
        • Cell proliferation
        • Cell viability and apoptosis
        • DNA content
        • Cell cycle.

        The Telethon Kids Flow Facility is equipped with 3 flow cytometers/analysers, one cell sorter and one cell separator.

        • BD Symphony A5SE
          • 355nm (UV) 405nm (Violet), 488nm (Blue), 561nm (Yellow/Green) and 640nm (Red) excitation lasers
          • 48 fluorescence parameters
          • Enables both spectral unmixing and compensation workflows
          • High-Sensitivity GaAs PMTs Enables square form fit and gallium arsenide (GaAs) PMTs for enhanced resolution
          • HTS sampling from 96 and 384 well plates
          • Algorithmically Optimized Filter Set
          • BD FACS Diva Software
        • BD LSR Fortessa
          • 355nm (UV) 405nm (Violet), 488nm (Blue), 561nm (Yellow/Green) and 640nm (Red) excitation lasers
          • 18 fluorescence parameters
          • HTS sampling from 96 and 384 well plates
          • BD FACS Diva Software
        • BD LSR Fortessa X-20
          • 355nm (UV) 405nm (Violet), 488nm (Blue), 561nm (Yellow/Green) and 640nm (Red) excitation lasers
          • 18 fluorescence parameters
          • HTS sampling from 96 and 384 well plates
          • BD FACS Diva Software
        • Flow Cytometer Sorters

          BD FACSAria Fusion Cell Sorter

          • 355nm (UV) 405nm (Violet), 488nm (Blue), 561nm (Yellow/Green) and 640nm (Red) excitation lasers
          • 18 fluorescence parameters
          • BD FACS Diva Software
          • 4-way sorting with multiple adaptors/devices
          • 70u, 85u, 100u Nozzle size option 

          BD FACS Melody Cell Sorter

          • 405nm (Violet), 488nm (Blue), and 640nm (Red) excitation lasers
          • 9 fluorescence parameters
          • Fixed 100u nozzle size
          • FACS Chorus Software
          • 4-way sorting with multiple adaptors/devices
          • Automated stream setup and steering into tubes or plates saves time
             

        Our laboratory technologies

        Contact us

        Please direct general enquiries to our reception on (08) 6319 1000. For further information about our laboratory equipment, please contact:

        Pradeep Kumar
        Flow Facility & Equipment Specialist
        Phone: (08) 6319 1433
        Email: Pradeep Kumar

        Stephen Proksch
        Flow Facility & Equipment Technician
        Phone: (08) 6319 1434
        Email: Stephen Proksch